In vitro anti–Candida albicans activity of 100% peppermint (Mentha piperita) extract compared to 5% sodium hypochlorite in acrylic dentures
DOI:
https://doi.org/10.24265/kiru.2026.v23n1.05Keywords:
Sodium Hypochlorite; Mentha; Candida albicans; Antifungal agents; Mentha piperitaAbstract
Objective: To evaluate the in vitro anti-Candida albicans effect of 100% peppermint extract compared with 5% sodium hypochlorite on acrylic dental prostheses. Materials and Methods: This in vitro experimental analysis was carried out with 30 pieces of thermocured acrylic resin, 15 mm long x 15 mm thick, manufactured with attributes similar to those of a partially removable acrylic denture. The polymerization times and polishing procedures set by the manufacturer were strictly adhered to. sample processing and analysis were perfomed at the Microbiology Laboratory of the Academic Health and Wellness Unit at the Azogues campus of the Catholic University of Cuenca. Results: Both the 100% peppermint extract and 5% sodium hypochlorite were shown to inhibit the growth of Candida albicans after 15 minutes of exposure. Conclusions: The 100% peppermint extract and 5% sodium hypochlorite have antifungal activity in inhibiting the growth of Candida albicans on acrylic dental prostheses.
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Philbert R, Shimba E, Muro F, Rwakatema D. Oral prosthesis cleaning practice and oral health status of removable oral prosthesis wearers attended Kilimanjaro Christian Medical Centre, Moshi, Tanzania. Tanzania J Health Res. 2025;26(3):2055-64.
San Martín Andrade DF, González Andrade FN, Calderón Alemán DE. Estudio in vitro del efecto antifúngico en prótesis removibles acrílicas colonizadas por Candida albicans de 3 desinfectantes comerciales y 3 plantas medicinales. En: Investigación Contemporánea desde una Visión Multidisciplinar. Libro 6. Ciencias de la Salud. Quito (EC): Red Latinoamericana de Investigación Contemporánea (RedLIC); 2024. doi: 10.58995/lb.redlic.40.273.
Mohamed SZ, Ismael NS, Rokaya D, Alghonemy WY, Tawfik EA, Atia GAN. Efficacy of Three Mouthwashes on Candida albicans in Patients with Denture Stomatitis: In Vivo Study. Open Dent J. 2025;19:e18742106376765. doi: 10.2174/0118742106376765250602104503.
Perić M, Miličić B, Kuzmanović Pfićer J, Živković R, Arsić Arsenijević V. A Systematic Review of Denture Stomatitis: Predisposing Factors, Clinical Features,
Etiology, and Global Candida spp. Distribution. J Fungi. 2024;10(5):328. doi:10.3390/jof10050328.
Abuhajar E, Ali K, Zulfiqar G, Al Ansari K, Raja HZ, Bishti S, et al. Management of Chronic Atrophic Candidiasis (Denture Stomatitis)—A Narrative Review. Int J Environ Res Public Health. 2023;20(4):3029. doi: 10.3390/ijerph20043029.
Patel M. Oral Cavity and Candida albicans: Colonisation to the Development of Infection. Pathogens. 2022;11(3):335. doi: 10.3390/pathogens11030335.
Carretto C de FP, Junqueira JC, Almeida RB de A, Furlan MR, Jorge AOC. Antimicrobial activity of Mentha piperita L. against Candida spp. Braz Dent Sci. 2011;13(1/2):4–9. doi: 10.14295/bds.2010.v13i1/2.160.
Ribeiro AB, Pizziolo PG, Clemente LM, Aguiar HC, Poker B de C, Silva AAM e, et al. Strategies for Preventing and Treating Oral Mucosal Infections Associated with Removable Dentures: A Scoping Review. Antibiotics. 2024;13(3):273. doi: 10.3390/antibiotics13030273.
Mylonas P, Milward P, McAndrew R. Denture cleanliness and hygiene: an overview. Br Dent J. 2022;233(1):20–6. doi: 10.1038/s41415-022-4397-1.
Salles MM, Badaró MM, Arruda CNF, Leite VMF, Silva CHL, Watanabe E, et al. Antimicrobial activity of complete denture cleanser solutions based on sodium hypochlorite and Ricinus communis – a randomized clinical study. J Appl Oral Sci. 2015;23(6):637-42. doi: 10.1590/1678-775720150204.
Namala B, Hegde V. Comparative evaluation of the effect of plant extract, Thymus vulgaris and commercially available denture cleanser on the flexural strength and surface roughness of denture base resin. J Indian Prosthodont Soc. 2019;19(3):261. doi: 10.4103/jips.jips_141_19.
Dhivya M, Nandini VV. Does Mint have Antifungal and Antibacterial effect. Int J Health Sci (Qassim). 2022;1536–42. doi: 10.53730/ijhs.v6nS5.8912.
Wenji KY, Rukmi I, Suprihadi A. In vitro Antifungal Activity of Methanolic and Chloroform Mint Leaves (Mentha piperita L.) Extracts Against Candida albicans. J Phys Conf Ser. 2019;1217(1):012136. doi: 10.1088/1742-6596/1217/1/012136.
Guallpa KM, Santana K, Chamba SF, San Martin Andrade D, Calderón Alemán DE, Medina-Sotomayor P. Eficacia de desinfectantes naturales contra Candida albicans en prótesis dental parcial y total. Kiru. 2024;21(2):84–90. doi: 10.24265/kiru.2024.v21n2.06.
Ghaznavi A, Pishva SS, Heydarlou MJ. Phytotherapy in oral candidiasis: A review of the most important native Iranian medicinal plants effective against Candida albicans, the cause of oral thrush. A review. Rev Bras Hig Sanid Anim. 2024;18(4):1–16. doi: 10.5935/1981-2965.20240027.
Rajkowska K, Otlewska A, Kunicka-Styczyńska A, Krajewska A. Candida albicans Impairments Induced by Peppermint and Clove Oils at Sub-Inhibitory Concentrations. Int J Mol Sci. 2017;18(6):1307. doi: 10.3390/ijms18061307.
Kaypetch R, Rudrakanjana P, Tua-ngam P, Tosrisawatkasem O, Thairat S, Tonput P, et al. Effects of two novel denture cleansers on multispecies microbial biofilms, stain removal and the denture surface: an in vitro study. BMC Oral Health. 2023;23(1):852. doi: 10.1186/s12903-023-03535-5.
Anjum R, Dhaded S, Joshi S, Sajjan C, Konin P, Reddy Y. Effect of plant extract denture cleansing on heat-cured acrylic denture base resin: An in vitro study. J Indian Prosthodont Soc. 2017;17(4):401. doi: 10.4103/jips.jips_97_17.
Ribeiro AB, Pizziolo PG, Clemente LM, Aguiar HC, Poker B de C, Silva AAM e, et al. Strategies for Preventing and Treating Oral Mucosal Infections Associated with Removable Dentures: A Scoping Review. Antibiotics. 2024;13(3):273. doi: 10.3390/antibiotics13030273.
Calderón Alemán DE, San Martín Andrade DF, Carrión Cueva AP, Medina-Sotomayor P, Montesinos-Rivera V, Tello Larriva MP. Eficacia de tres agentes desinfectantes para prótesis acrílicas colonizadas por Candida albicans: un estudio in vitro. Arch Venez Farmacol Ter. 2022;41(10):709-713.
San Martin D, Castro Navarrete L, Palacios Machuca V, Medina Sotomayor P. Métodos de desinfección para prótesis removible colonizadas por cándida SPP. Revista Científica Especialidades Odontológicas UG. 2023;6(1):66–74. doi: 10.53591/eoug.v6i1.1622.
De Sousa Porta SR, de Lucena‐Ferreira SC, da Silva WJ, Del Bel Cury AA. Evaluation of sodium hypochlorite as a denture cleanser: a clinical study. Gerodontology. 2015;32(4):260–6. doi: 10.1111/ger.12104.
Amaya Arbeláez MI, Vergani CE, Barbugli PA, Pavarina AC, Sanitá PV, Jorge JH. Long-term effect of daily chemical disinfection on surface topography and Candida albicans biofilm formation on denture base and reline acrylic resins. Oral Health Prev Dent. 2020;18:999–1010.
Alvarez Chupillón H, Portocarrero-Mondragón J, Pardo-Aldave K. Comparación de la actividad antifúngica in vitro del gluconato de clorhexidina 2% e hipoclorito de sodio 5,25% contra Candida albicans. Av Odontoestomatol. 2022 Jun;38(2):85–9. doi: 10.4321/S0213-12852022000200007.
Salloum AM. Effect of 5.25 % Sodium Hypochlorite on Color Stability of Acrylic and Silicone Based Soft Liners and a Denture Base Acrylic Resin. J Indian Prosthodont Soc. 2014;14(2):179–86. doi: 10.1007/s13191-013-0309-z
Vásquez Lizama G, Ramos Pazos N, Yefi Carrasco R. Desinfectantes convencionales y alternativas sobre el desarrollo de cándida albicans. TECHNO REVIEW International Technology, Science and Society Review Rev Int Tecnol Cienc Soc. 2023;13(3):1–12. doi:10.37467/revtechno.v13.4807……………………………
Sampath A, Velaga P, Devarapalli S, Guntupalli S, Peeta RB, Boyapati H. Impact of Chemical Disinfectants on Surface Microhardness of Acrylic Resin Denture Teeth: An In Vitro Study. Cureus. 2025;17(7):e88801. doi: 10.7759/cureus.88801.
González Ríos P, Abarzúa Ortega K, Correa Leiva N. Análisis de la efectividad de limpiadores protésicos en la remoción de Candida albicans presente en prótesis total superior. Rev Conflu. 2025 Jan 21;8. doi: 10.52611/confluencia.2025.1234.
Yuzugullu B, Acar O, Cetinsahin C, Celik C. Effect of different denture cleansers on surface roughness and microhardness of artificial denture teeth. J Adv Prosthodont. 2016;8(5):333. doi: 10.4047/jap.2016.8.5.333
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Copyright (c) 2026 Kevin Michael Guallpa Ochoa , Daniela Fernanda San Martin Andrade, Janneth Cecilia Urgiles Esquivel , Ivan Bolívar Valladolid Zhizhingo
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